Difference between revisions of "Part:BBa K1604019"

 
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<partinfo>BBa_K1604019 short</partinfo>
 
<partinfo>BBa_K1604019 short</partinfo>
  
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This part contains RBS and blh gene.
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This part contains RBS and blh gene. &#946;-carotene 15,15&rsquo;-dioxygenase is involved in retinal biosynthesis and catalizes the last step of the  pathway by cleaving one molecule of &#946;-carotene into two molecules of retinal.  Blh is the gene that encodes for this enzyme, and was first isolated in the uncultured bacteria SAR86 <html><a href="#fn:1" id="fnref:1" title="see footnote" class="footnote">[1]</a></html> . Blh is present in other organisms (i.e. cyanobacteria and algae) however enzymes involved in the cleavage of &#946;-carotene that involve different mechanisms are found in many other organisms.
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The enzyme requires oxygen for the cleavage of &#946;-carotene, which was demonstrated to be provided by water <html><a href="#fn:2" id="fnref:2" title="see footnote" class="footnote">[2]</a></html> . This data are consistent with the fact the bacteria lives in deep marine waters. It requires Fe2+ that is coordinated by 4 His residues that are highly conserved.
  
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===Usage and Biology===
 
===Usage and Biology===
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We placed BBa_K1604019 under a costituve promoter and we characterized blh. <br>
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To see the characterization results check the part <html><a href="https://parts.igem.org/Part:BBa_K1604021#Usage_and_Biology">BBa_K1604021</a></html> and the part <html><https://parts.igem.org/wiki/index.php?title=Part:BBa_K1604022">BBa_K1604022</a></html>
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<div class="footnotes">
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<hr />
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<ol>
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<li id="fn:1">
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<p>. Martinez A.,"Proteorhodopsin Photosystem Gene Expression Enables  Photophosphorylation in a Heterologous Host" Proceedings of the National Academy of Sciences 104.13 (2007): 5590-595. Web</p>
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</li>
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<li id="fn:2">
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<p>Yeong-Su Kim, Retinal production from b-carotene by b-carotene 15,150-dioxygenase from an uncµlturable marine bacterium, Biotechnol Lett (2010) 32:957–961</p>
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</li>
  
 
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Revision as of 16:50, 16 September 2015

RBS + β-carotene 15,15'-dioxygenase (blh gene)

This part contains RBS and blh gene. β-carotene 15,15’-dioxygenase is involved in retinal biosynthesis and catalizes the last step of the pathway by cleaving one molecule of β-carotene into two molecules of retinal. Blh is the gene that encodes for this enzyme, and was first isolated in the uncultured bacteria SAR86 [1] . Blh is present in other organisms (i.e. cyanobacteria and algae) however enzymes involved in the cleavage of β-carotene that involve different mechanisms are found in many other organisms.

The enzyme requires oxygen for the cleavage of β-carotene, which was demonstrated to be provided by water [2] . This data are consistent with the fact the bacteria lives in deep marine waters. It requires Fe2+ that is coordinated by 4 His residues that are highly conserved.

Usage and Biology

We placed BBa_K1604019 under a costituve promoter and we characterized blh.
To see the characterization results check the part BBa_K1604021 and the part BBa_K1604022


  1. . Martinez A.,"Proteorhodopsin Photosystem Gene Expression Enables Photophosphorylation in a Heterologous Host" Proceedings of the National Academy of Sciences 104.13 (2007): 5590-595. Web

  2. Yeong-Su Kim, Retinal production from b-carotene by b-carotene 15,150-dioxygenase from an uncµlturable marine bacterium, Biotechnol Lett (2010) 32:957–961

    3. Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal NgoMIV site found at 202
    • 1000
      COMPATIBLE WITH RFC[1000]