Difference between revisions of "Part:BBa K1679007"

Revision as of 14:56, 16 September 2015

promoter+RNA thermometer+RFP

This is a device containing a promoter (BBa_J23101),a RNA thermometer (BBa_K115001) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli. RNA Thermometers(RNAT) are temperature-sensing RNA sequences in 5’UTR of their mRNAs. At low temperature, RNAT folds into structure, blocking access of ribosome; At high temperature, RNAT switch from off to open conformation, increasing the efficiency of translation initiation. When the temperature rises above 42°C, RFP would be expressed in theory.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 700
Illegal AgeI site found at 812
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1679007 short</partinfo>
-This is a device containing a promoter (BBa_J23101),a RBS (BBa_K115001) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.In theory,When the temperature rises above 42°C,It can produce RFP.However,according to our experimental,It can't appear red.
+This is a device containing a promoter (BBa_J23101),a RNA thermometer (BBa_K115001) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.
+RNA Thermometers(RNAT) are temperature-sensing RNA sequences in 5’UTR of their mRNAs. At low temperature, RNAT folds into structure, blocking access of ribosome; At high temperature, RNAT switch from off to open conformation, increasing the efficiency of translation initiation.
+When the temperature rises above 42°C, RFP would be expressed in theory.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1679007 SequenceAndFeatures</partinfo>