Difference between revisions of "Part:BBa K1767005"

Revision as of 14:48, 16 September 2015

P(Lac)IQ RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter

We designed and built a kill switch mechanism that based on the activation of a lethal gene downstream of an inducible promoter that is activated over time.

This is the full construct of our genetic circuit without AiiA which consists of a constitutive promoter (I14032) driving expression of LuxR followed by Lux promoter (R0062). This promoter is activated by the LuxR-AHL complex , driving expression of tetR , followed by a Tet promoter (R0040) . This promoter is repressed by TetR protein , driving expression of a fluorescent protein, in this case RFP (E1010).

We built this BioBrick for testing how our construct behaves without AiiA , to compare this BioBrick that lacks AiiA with the construct that contains AiiA (BBa_k1767003).We wanted to investigate how the BioBrick behaves and works and to check how long the fluorescent protein (RFP) is expressed when we lack the protein that degrades the inducer (AHL).

The scheme below explains how this BioBrick works :

We performed an experiment to test this part, and here is the result:

Sequence and Features