Difference between revisions of "Part:BBa K1655001"
| Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
| + | |||
| + | |||
<partinfo>BBa_K1655001 short</partinfo> | <partinfo>BBa_K1655001 short</partinfo> | ||
| Line 7: | Line 9: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
This part sequence is derived from https://parts.igem.org/Part:BBa_I13504. The part has been PCRed from BBa_I13504, and the reverse primer has added an extra guanine nucleotide prior to the birck's suffix. This will allow for easy aminoterminal GFP fusion with the biobrick enzyme assembly. | This part sequence is derived from https://parts.igem.org/Part:BBa_I13504. The part has been PCRed from BBa_I13504, and the reverse primer has added an extra guanine nucleotide prior to the birck's suffix. This will allow for easy aminoterminal GFP fusion with the biobrick enzyme assembly. | ||
| − | + | ||
| − | + | ||
| − | + | ||
Revision as of 14:22, 16 September 2015
This GFP can be fused into any protein's aminoterminal end with the BioBrick enzyme assembly method.
An extra nucelotide has been added prior to this GFP brick's suffix to maintain the reading frame after fusion.