Difference between revisions of "Part:BBa K1679004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We want to make a promoter part containing RiboJ to buffer synthetic circuits from genetic context. We synthesized RiboJ with EXSP site and use 3A Assembly to construct this part. | + | After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme with an additional 23-nt hairpin immediately downstream to help expose the RBS. We want to make a promoter part containing RiboJ to buffer synthetic circuits from genetic context. We synthesized RiboJ with EXSP site and use 3A Assembly to construct this part. |
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===Source=== | ===Source=== | ||
Latest revision as of 13:55, 16 September 2015
J23101 and RiboJ
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme with an additional 23-nt hairpin immediately downstream to help expose the RBS. We want to make a promoter part containing RiboJ to buffer synthetic circuits from genetic context. We synthesized RiboJ with EXSP site and use 3A Assembly to construct this part.
Source
BBa_J23101 We synthesized RiboJ. Our reference is Lou C, Stanton B, Chen Y J, et al. Ribozyme-based insulator parts buffer synthetic circuits from genetic context[J]. Nature biotechnology, 2012, 30(11): 1137-1142.
References
Lou C, Stanton B, Chen Y J, et al. Ribozyme-based insulator parts buffer synthetic circuits from genetic context[J]. Nature biotechnology, 2012, 30(11): 1137-1142.