Difference between revisions of "Part:BBa I13001"

Revision as of 09:53, 16 September 2015

RBS B0030 w/ YFP (-LVA) TT

B0030. E0030. B0015

Glasgow iGEM 2011

This part was found to express well when excited with fluorescence in the range of 515-565nm. See images below for expression testing:

Shenzhen_SFLS (IGEM 2015)

We expressed this part in E.coli using pbad promoter and J23116 promoter.

From left to right: J23116/pbad(0μM L-Arabinose)/1.0μM/2.0μM/4.0μM(best in the 5 concentrations we usd)/8.0μM

Sequence and Features

Assembly Compatibility:

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	From left to right: J23116/pbad(0μM L-Arabinose)/1.0μM/2.0μM/4.0μM(best in the 5 concentrations we usd)/8.0μM		From left to right: J23116/pbad(0μM L-Arabinose)/1.0μM/2.0μM/4.0μM(best in the 5 concentrations we usd)/8.0μM
−	[[File:QQ图片20150210175650~~.jpeg~~.jpg]] [[File:QQ图片20150210175655~~.jpeg~~.jpg]]	+	[[File:QQ图片20150210175650.jpg]] [[File:QQ图片20150210175655.jpg]]