Difference between revisions of "Part:BBa I13001"

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From left to right: J23116/pbad(0μM L-Arabinose)/1.0μM/2.0μM/4.0μM(best in the 5 concentrations we usd)/8.0μM
 
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[[File:QQ图片20150210175650.jpeg]] [[File:QQ图片20150210175655.jpeg]]  
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Revision as of 09:51, 16 September 2015

RBS B0030 w/ YFP (-LVA) TT

B0030. E0030. B0015


Experience

Glasgow iGEM 2011

This part was found to express well when excited with fluorescence in the range of 515-565nm. See images below for expression testing:

Gla YFP Results.jpg


Shenzhen_SFLS (IGEM 2015)

We expressed this part in E.coli using pbad promoter and J23116 promoter.

From left to right: J23116/pbad(0μM L-Arabinose)/1.0μM/2.0μM/4.0μM(best in the 5 concentrations we usd)/8.0μM File:QQ图片20150210175650.jpeg File:QQ图片20150210175655.jpeg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]