Difference between revisions of "Part:BBa K1632013"

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FimE is Fim recombinase.<br>
 
FimE is Fim recombinase.<br>
With the expression of FimE, the <i>fim</i> switch only inverts from the ON state to OFF state.<br>
+
With the expression of FimE, the <i>fim</i> switch (example <partinfo>BBa_K1632000</partinfo>, <partinfo>BBa_K1632004</partinfo> etc...) only inverts from the ON state to OFF state.<br>
 
The expression of this Fim recombinase is controlled by arabinose in BBa_K1632013.
 
The expression of this Fim recombinase is controlled by arabinose in BBa_K1632013.
  

Revision as of 09:20, 16 September 2015

PBAD/araC_rbs_fimE(wild-type)

FimE is Fim recombinase.
With the expression of FimE, the fim switch (example BBa_K1632000, BBa_K1632004 etc...) only inverts from the ON state to OFF state.
The expression of this Fim recombinase is controlled by arabinose in BBa_K1632013.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1260
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961