Difference between revisions of "Part:BBa K1653006"

Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1653006 short</partinfo>
 
<partinfo>BBa_K1653006 short</partinfo>
 +
<br>
 
We improved the characterization of a previously existing BioBrick Part [https://parts.igem.org/Part:BBa_K1230000 BBa_K1230000] and submitted this improved BioBrick ''marA'' device as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1653006 BBa_K1653006] to iGEM Registry.
 
We improved the characterization of a previously existing BioBrick Part [https://parts.igem.org/Part:BBa_K1230000 BBa_K1230000] and submitted this improved BioBrick ''marA'' device as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1653006 BBa_K1653006] to iGEM Registry.
 
In exsisting part's information of ''marA'', it gives ''E. coli'' resistance against kanamycin only.  
 
In exsisting part's information of ''marA'', it gives ''E. coli'' resistance against kanamycin only.  

Revision as of 04:30, 16 September 2015

MarA
We improved the characterization of a previously existing BioBrick Part BBa_K1230000 and submitted this improved BioBrick marA device as BBa_K1653006 to iGEM Registry. In exsisting part's information of marA, it gives E. coli resistance against kanamycin only.
In this year, we confilmed that overepressing of marA gives E. coli resistance against geraniol as one of the terpene and decrease its intracellular concentration. This information is very beneficial for other iGEMers to production of organic substance that have toxicity using bacteria. [here]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]