Difference between revisions of "Part:BBa K1682016:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | A strong constiuitive promoter was used to drive the expression of Lacl, this may had lead to a lower dynamic range of the system in response to IPTG (1), which lead to difficulty in RFP output measurement. The dynamic range and sensitivity possibly be increased by choice of a weaker constituitive promoter. | ||
+ | 1. Wang, B., Barahona, M., & Buck, M. (2015). Amplification of small molecule-inducible gene expression via tuning of intracellular receptor densities. Nucleic Acids Research, 43(3), 1955-1964 | ||
===Source=== | ===Source=== |
Latest revision as of 03:49, 16 September 2015
IPTG induced RFP reporter system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1084
Illegal NheI site found at 1107 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2285
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 781
Illegal AgeI site found at 893 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
A strong constiuitive promoter was used to drive the expression of Lacl, this may had lead to a lower dynamic range of the system in response to IPTG (1), which lead to difficulty in RFP output measurement. The dynamic range and sensitivity possibly be increased by choice of a weaker constituitive promoter.
1. Wang, B., Barahona, M., & Buck, M. (2015). Amplification of small molecule-inducible gene expression via tuning of intracellular receptor densities. Nucleic Acids Research, 43(3), 1955-1964
Source
Biobrick