Difference between revisions of "Part:BBa K1688007"
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Revision as of 20:12, 15 September 2015


ModLac laccase with His-tag and HlyA export tag (inc RBS and J23110 promoter)

Assembly of Anderson promoter BBa_J23110 and RBS + ModLac with HlyA tag (BBa_K1688006). ModLac is a modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 310
  • 1000
    COMPATIBLE WITH RFC[1000]