Difference between revisions of "Part:BBa K1766008"
Line 3: Line 3:
  
 
EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in <i>E.coli</i>. Structure includes the EnvZ247 allele [1] which is linked through a GGSSAAG linker to a V5 epitope tag. The full structure has a predicted size of 115.36 kDa [2]. The part was provided in the pRD400 (or pEnvZ) backbone under a lac-inducible promoter [3].
 
EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in <i>E.coli</i>. Structure includes the EnvZ247 allele [1] which is linked through a GGSSAAG linker to a V5 epitope tag. The full structure has a predicted size of 115.36 kDa [2]. The part was provided in the pRD400 (or pEnvZ) backbone under a lac-inducible promoter [3].
−
<br />  
+
<br /> <br />
−
Two-component regulatory systems are a common in procaryotes for signal transduction through the membrane. In this case, EnvZ is activated by changes in osmotic pressure leading to phosphorylation of the kinase. The response regulator OmpR is then activated throungh phosphorylation resultning in the regulation of expression of the outer membrane porin proteins OmpF and OmpC.  
+
 
 +
==Biology==
 +
Two-component regulatory systems are a common in procaryotes for signal transduction through the membrane. In this case, EnvZ is activated by changes in osmotic pressure leading to phosphorylation of the kinase. The response regulator OmpR is then activated through phosphorylation resulting in the regulation of expression of the outer membrane porin proteins OmpF and OmpC.
 +
 
 +
==Usage==
 +
EnvZ was used primarily as a scaffold for creating the bacterial antigen receptors: BAR1 (BBa_K1766009), BAR2 (BBa_K1766010) and BAR3 (BBa_K1766011). Besides this, EnvZ was also used as a control for many of the experiments with the BAR constructs.  
  
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1766008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1766008 SequenceAndFeatures</partinfo>
  
−
<!-- Add more about the biology of this part here -->
 
 
===Experiments===
 
===Experiments===
 
<b>Experssion</b>
 
<b>Experssion</b>
 
<br />
 
<br />
 +
  
 
<b>Osmolarity tests</b>
 
<b>Osmolarity tests</b>

Revision as of 17:09, 15 September 2015

EnvZ_V5 osmoregulatory histidine kinase from E.coli.

EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in E.coli. Structure includes the EnvZ247 allele [1] which is linked through a GGSSAAG linker to a V5 epitope tag. The full structure has a predicted size of 115.36 kDa [2]. The part was provided in the pRD400 (or pEnvZ) backbone under a lac-inducible promoter [3].

Biology

Two-component regulatory systems are a common in procaryotes for signal transduction through the membrane. In this case, EnvZ is activated by changes in osmotic pressure leading to phosphorylation of the kinase. The response regulator OmpR is then activated through phosphorylation resulting in the regulation of expression of the outer membrane porin proteins OmpF and OmpC.

Usage

EnvZ was used primarily as a scaffold for creating the bacterial antigen receptors: BAR1 (BBa_K1766009), BAR2 (BBa_K1766010) and BAR3 (BBa_K1766011). Besides this, EnvZ was also used as a control for many of the experiments with the BAR constructs.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]

Experiments

Experssion


Osmolarity tests


References

[1] Russo, F. D. (1992) Ph.D. thesis. Princeton University, Princeton, N.J.
[2] Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A. (2005) Protein Identification and Analysis Tools on the ExPASy Server; (In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press
[3] Nørholm M. H. H., von Heijne G., and Draheim R. R. (2014) Forcing the Issue: Aromatic Tuning Facilitates Stimulus-Independent Modulation of a Two-Component Signaling Circuit. ACS Synth. Biol. 2015, 4, 474−481