Difference between revisions of "Part:BBa K1675001:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1675001 short</partinfo>
 
<partinfo>BBa_K1675001 short</partinfo>
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===Design Notes===
 
===Design Notes===
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mutation of CTG to CTA Leu.(site:336bp); mutation of GCT to GCG Ala.(site:414bp); mutation of ACC to ACT Thr.(site:519bp); mutation of GAC to GAT Asp.(site:525bp); mutation of ACC to ACT Thr.(site:630bp); mutation of GTA to GTG Val.(site:645bp); mutation of GCA to GCG Ala.(site:993bp); mutation of GAG to GAA Glu.(site:1020bp); mutation of GTC to GTA Val.(site:1452bp)
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None.
  
  

Latest revision as of 16:43, 15 September 2015

NhaB; sodium-proton antiporter; membrane protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1003
    Illegal AgeI site found at 1315
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.



Source

Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=1233176&to=1234717&strand=true

References