Difference between revisions of "Part:BBa K1648004"
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[[File:K1648004.png|350px|thumb|right|Construct Map for K1648004]]
 
[[File:K1648004.png|350px|thumb|right|Construct Map for K1648004]]
  
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Consist of six fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome.
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Consist of a genome (mamC) from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome.
  
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Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamXY, mamGFDC and mms6 operons''' except the mamC operon is designed for '''homologous recombination'''.
 
  
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Part containing this template ([https://parts.igem.org/Part:BBa_K1648002 K1648002]) together with another part containing mamC ([https://parts.igem.org/Part:BBa_K1648XXX K1648XXX]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:51, 15 September 2015

Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane

Construct Map for K1648004

Consist of a genome (mamC) from magnetosome island of Magnetospirillum gryphiswaldense genome.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2759
    Illegal XbaI site found at 2765
    Illegal SpeI site found at 1
    Illegal PstI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2759
    Illegal SpeI site found at 1
    Illegal PstI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2759
    Illegal XhoI site found at 1162
    Illegal XhoI site found at 2188
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2759
    Illegal XbaI site found at 2765
    Illegal SpeI site found at 1
    Illegal PstI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2759
    Illegal XbaI site found at 2765
    Illegal SpeI site found at 1
    Illegal PstI site found at 7
    Illegal NgoMIV site found at 2546
    Illegal NgoMIV site found at 2636
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2611