Difference between revisions of "Part:BBa K1655001"
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<partinfo>BBa_K1655001 short</partinfo> | <partinfo>BBa_K1655001 short</partinfo> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | This part sequence is derived from https://parts.igem.org/Part:BBa_I13504. The part has been PCRed from BBa_I13504, and the reverse primer has added an extra guanine nucleotide prior to the birck's suffix. This will allow for easy aminoterminal GFP fusion with the biobrick enzyme assembly. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 15:18, 15 September 2015
This GFP can be fused into any protein's aminoterminal end with the BioBrick enzyme assembly method.
An extra nucelotide has been added prior to this GFP brick's suffix to maintain the reading frame after fusion.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644