Difference between revisions of "Part:BBa K1648002"

Revision as of 15:01, 15 September 2015

Recombination Template for mamAB Operon with Promotor and Terminator

Construct Map for K1648002

Consist of a template for recombination of mamAB operon(K1648000), a promotor & RBS(J13002) in front of each template and a double terminator(B0015) at the back of each template.

This part is designed for homologous recombination to introduce the large magnetosome forming operon into Azotobactor vinelandii.

This part is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 855
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 371
Illegal AgeI site found at 890
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 616

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1648002 short</partinfo>
 [[File:K1648002_Map.png|350px|thumb|right|Construct Map for K1648002]]
-Consist of a template for recombination of <b> mamAB </b> operon([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1648000 K1648000]), a template for recombination of<b> mamXY, mamGC and mms</b> operon([https://parts.igem.org/Part:BBa_K1648001:Design K1648001]), a promotor & RBS([https://parts.igem.org/Part:BBa_J13002 J13002]) in front of each template and a double terminator([https://parts.igem.org/Part:BBa_B0015 B0015]) at the back of each template.
+Consist of a template for recombination of <b> mamAB </b> operon([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1648000 K1648000]), a promotor & RBS([https://parts.igem.org/Part:BBa_J13002 J13002]) in front of each template and a double terminator([https://parts.igem.org/Part:BBa_B0015 B0015]) at the back of each template.
 This part is designed for <b>homologous recombination</b> to introduce the large magnetosome forming operon into <i>Azotobactor vinelandii</i>.