Difference between revisions of "Part:BBa K1675000"
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The gene, nhaA, is the archetypal Na+/H+ anti-porter in E. coli. It is the only member of the family that is absolutely required by E. coli for survival in high-salt conditions, under alkaline stress, or in the presence of otherwise toxic Li+ concentration. | The gene, nhaA, is the archetypal Na+/H+ anti-porter in E. coli. It is the only member of the family that is absolutely required by E. coli for survival in high-salt conditions, under alkaline stress, or in the presence of otherwise toxic Li+ concentration. | ||
NhaA catalyzes the exchange of 2 H+ per Na+. Therefore, nhaA could make sure E. coli’s survival under alkaline pH, which are in desperate need in our project. | NhaA catalyzes the exchange of 2 H+ per Na+. Therefore, nhaA could make sure E. coli’s survival under alkaline pH, which are in desperate need in our project. | ||
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+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
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+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K1675003 SequenceAndFeatures</partinfo> | ||
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+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K1675003 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 13:16, 15 September 2015
glutamate decarboxylase A (GadA)
The gene, nhaA, is the archetypal Na+/H+ anti-porter in E. coli. It is the only member of the family that is absolutely required by E. coli for survival in high-salt conditions, under alkaline stress, or in the presence of otherwise toxic Li+ concentration. NhaA catalyzes the exchange of 2 H+ per Na+. Therefore, nhaA could make sure E. coli’s survival under alkaline pH, which are in desperate need in our project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 640
Illegal AgeI site found at 700 - 1000COMPATIBLE WITH RFC[1000]