Difference between revisions of "Part:BBa K1723001"
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PAM rich URS J23117 is a promoter made from the existing J23117 (BBa_J23117) weak promoter. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA: Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004). | PAM rich URS J23117 is a promoter made from the existing J23117 (BBa_J23117) weak promoter. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA: Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004). | ||
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Revision as of 12:38, 15 September 2015
PAM rich URS j23117 promoter
PAM rich URS J23117 is a promoter made from the existing J23117 (BBa_J23117) weak promoter. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA: Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 56
Illegal NheI site found at 79 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 43
- 1000COMPATIBLE WITH RFC[1000]