Difference between revisions of "Part:BBa K1723001"

Revision as of 11:52, 15 September 2015

PAM rich URS j23117 promoter

PAM rich URS J23117 is a promoter made from the existing J23117 (BBa_J23117) weak promoter. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA: Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004). All this system is operating in the special cell strain JEN202. In our experiments this promoter is 27 base pairs upstream the ATG start codon.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 56
Illegal NheI site found at 79
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 43
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1723001 short</partinfo>
-PAM rich URS J23117 is a promoter made from the existing J23117 (BBa_J23117) weak promoter. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-&#969; (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004).
+PAM rich URS J23117 is a promoter made from the existing J23117 (BBa_J23117) weak promoter. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-&#969; (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA: Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004).
 All this system is operating in the special cell strain JEN202. In our experiments this promoter is 27 base pairs upstream the ATG start codon.