Difference between revisions of "Part:BBa K1682016"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1682016 short</partinfo>
 
<partinfo>BBa_K1682016 short</partinfo>
  
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HKUST-RICE team designed an IPTG inducible RFP reporter system. We achieved this by combining constitutive promoter (BBa_J23101) with lacI generator (BBa_P0412), RFP coding device (BBa_J04450) driven by PlacI.
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HKUST-RICE team designed an IPTG inducible RFP reporter system. We achieved this by combining constitutive promoter <partinfo>BBa_J23101</partinfo> with LacI generator <partinfo>BBa_P0412</partinfo>, RFP coding device <partinfo>BBa_J04450</partinfo> driven by Plac.
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==Mechanism==
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[[File:HKUST-RICE BBa K1682016.1.jpg|500px|]]
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'''Figure 1. Schematic diagram of the inducible Plac-mRFP construct.''' '''(a)'''In the absence of Isopropyl b-D-1-thiogalactopyranoside (IPTG), LacI represses Plac. '''(b)'''When IPTG is present, LacI protein is repressed, triggering the production of mRFP.
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==Usage and Functionality==
  
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In order to check the functionality of the construct for use in sensing IPTG concentrations. Characterisation
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1682016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1682016 SequenceAndFeatures</partinfo>

Revision as of 08:17, 15 September 2015

IPTG induced RFP reporter system

HKUST-RICE team designed an IPTG inducible RFP reporter system. We achieved this by combining constitutive promoter BBa_J23101 with LacI generator BBa_P0412, RFP coding device BBa_J04450 driven by Plac.


Mechanism

HKUST-RICE BBa K1682016.1.jpg

Figure 1. Schematic diagram of the inducible Plac-mRFP construct. (a)In the absence of Isopropyl b-D-1-thiogalactopyranoside (IPTG), LacI represses Plac. (b)When IPTG is present, LacI protein is repressed, triggering the production of mRFP.


Usage and Functionality

In order to check the functionality of the construct for use in sensing IPTG concentrations. Characterisation

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1084
    Illegal NheI site found at 1107
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2285
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]