Difference between revisions of "Part:BBa K1592004"
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<partinfo>BBa_K1592004 short</partinfo> | <partinfo>BBa_K1592004 short</partinfo> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1592004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1592004 SequenceAndFeatures</partinfo> | ||
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| + | <h1>'''Usage'''</h1> | ||
| + | We use Php4d and construct circuits (Figure 1 and Figure 2) to express the cell surface display Si-tag system of Yarrowia Lipolytica (more experimental details see [https://parts.igem.org/Part:BBa_K1592007 BBa_K1592007]- [https://parts.igem.org/Part:BBa_K1592014 BBa_K1592014]) and the LIP2 prepro-Mcfp3([https://parts.igem.org/Part:BBa_K1592003 BBa_K1592003]) for validating the parts’ function. | ||
| + | [[File:HUST-China_2015_circuit_1.png|400px|thumb|center|Figure1: Php4d+LIP2 prepro-Mcfp+terminator]] | ||
| + | [[File:HUST-China_2015_circuit_2.png|400px|thumb|center|Figure2: Php4d+LIP2 prepro- Si-tag -YLcwp3 +terminator]] | ||
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Latest revision as of 07:40, 15 September 2015
promoter hp4d
The recombinant promotor hp4d, nearly unaffected by pH, carbon source, nitrogen source and some conditions, can strongly promote gene expression in any culture medium(Madzak et al., 2000). Promoter hp4d is composed of four tandem repeated UAS1 sequences in upstream and the small LEU2 promoter, which is the sequence between TATA box and start condon ATG of LEU2 promoter in downstream. UAS1 is the upsteam activating sequence of the pXPR2, which is less affected by other conditions. Though any inducers are non-essential for activiation of php4d during cultivating, hp4d, activated by unknown element, isn't constitutive promoter. The gene promoted by promoter hp4d usually expresses at the early stage of stabilization, called growth phase dependent (Nicaud et al., 2002). This feature, separating the strain growth and producing foreign protein, is useful for production of foreign protein, which avoids the harm by expressing foreign protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 92
Illegal BsaI.rc site found at 201
Illegal BsaI.rc site found at 310
Illegal BsaI.rc site found at 419
Usage
We use Php4d and construct circuits (Figure 1 and Figure 2) to express the cell surface display Si-tag system of Yarrowia Lipolytica (more experimental details see BBa_K1592007- BBa_K1592014) and the LIP2 prepro-Mcfp3(BBa_K1592003) for validating the parts’ function.
