Difference between revisions of "Part:BBa K1632013:Experience"
JunKawamura (Talk | contribs) (→Materials and Methods) |
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All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.<br> | All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.<br> | ||
| − | A. PBAD/araC_FimE(6A1) +fim switch[default ON]_gfp(3K3) <br> | + | A. PBAD/araC_FimE(wild-type) (6A1) +fim switch[default ON](wild-type)_gfp (3K3) <br> |
| − | B. PBAD/araC_FimE(6A1) +fim switch[default ON]_gfp(3K3) <br> | + | B. PBAD/araC_FimE(wild-type) (6A1) +fim switch[default ON](wild-type)_gfp (3K3) <br> |
| − | C. rbs_M256IcysE(6A1) + fim switch[default ON]_gfp(3K3) …positive control 1<br> | + | C. rbs_M256IcysE (6A1) + fim switch[default ON](wild-type)_gfp (3K3) …positive control 1<br> |
| − | D. rbs_M256IcysE(6A1) + fim switch[default OFF]_gfp(3K3) …negative control 1<br> | + | D. rbs_M256IcysE (6A1) + fim switch[default OFF](wild-type)_gfp (3K3) …negative control 1<br> |
| − | E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2 <br> | + | E. PBAD/araC_FimE(wild-type) (6A1) + J23119_gfp (3K3) …positive control 2 <br> |
| − | F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2 <br> | + | F. PBAD/araC_FimE(wild-type) (6A1)+rbs_gfp (3K3) …negative control 2 <br> |
[[Image:Tokyo_Tech_FimE_assay.png|thumb|center|600px|<b>Fig. 1. </b>Plasmids]]<br> | [[Image:Tokyo_Tech_FimE_assay.png|thumb|center|600px|<b>Fig. 1. </b>Plasmids]]<br> | ||
<b>2. Assay protocol</b><br> | <b>2. Assay protocol</b><br> | ||
Revision as of 07:12, 15 September 2015
PBAD/araC_rbs_fimE(wild-type)
Contents
Materials and Methods
1. Construction
All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.
A. PBAD/araC_FimE(wild-type) (6A1) +fim switch[default ON](wild-type)_gfp (3K3)
B. PBAD/araC_FimE(wild-type) (6A1) +fim switch[default ON](wild-type)_gfp (3K3)
C. rbs_M256IcysE (6A1) + fim switch[default ON](wild-type)_gfp (3K3) …positive control 1
D. rbs_M256IcysE (6A1) + fim switch[default OFF](wild-type)_gfp (3K3) …negative control 1
E. PBAD/araC_FimE(wild-type) (6A1) + J23119_gfp (3K3) …positive control 2
F. PBAD/araC_FimE(wild-type) (6A1)+rbs_gfp (3K3) …negative control 2
2. Assay protocol