Difference between revisions of "Part:BBa K1640018"

Revision as of 04:16, 15 September 2015

CHlM

ChlM gene from Chlamydomonas reinhardtii, encodes S-Adenosyl-L-methionine:magnesium protoporphyrin IX methyltransferase, a protein involved in chlorophyll biosynthesis.

Usage and Biology

The autotrophic gene ChlM is involved in the chlorophyll biosynthetic pathway in the formation of chlorophyll a (Meinecke et al. 2010). It encodes the enzyme S-Adenosyl-L-methionine:magnesium protoporphyrin IX methyltransferase (MgPMT), involved in the second step of chlorophyll biosynthesis from protoporphyrin IX (Block et al, 2002). MgPMT is accumulated through light activation, further activating genes aiding in the synthesis of chlorophyll a in the absence of light (Meinecke et al. 2010).

MgPMT catalyses the conversion of Mg-protoporphyrin IX through methyl group transfer forming Mg-protoporyphyrin IX monomethyl ester (Gibson et al., 1963).

S-adenosyl-L-methionine + magnesium protoporphyrin IX ⇌ S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester

This part allows E. coli to express the Chlamydomonas reinhardtii-derived ChlM gene involved in the chlorophyll biosynthesis pathway.

Sequence and Features