Difference between revisions of "Part:BBa K1640020:Design"
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===Design Notes===
 
===Design Notes===
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Modularisation: This part is to be combined with psbD, containing psbD, and psbC, containing psbC, to form psbDCA, one of five operons for the biosynthesis of a minimal Photosystem II.
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Overview: When induced, Escherichia coli will express the D1 subunit of the photosystem II reaction centre.
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Detection Methods: PsbMZH has been detected in E. coli by gel electrophoresis, and confirmed by DNA sequencing.
  
 
===Source===
 
===Source===

Revision as of 03:49, 15 September 2015

psbA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Modularisation: This part is to be combined with psbD, containing psbD, and psbC, containing psbC, to form psbDCA, one of five operons for the biosynthesis of a minimal Photosystem II.

Overview: When induced, Escherichia coli will express the D1 subunit of the photosystem II reaction centre.

Detection Methods: PsbMZH has been detected in E. coli by gel electrophoresis, and confirmed by DNA sequencing.

Source

Chlamydomonas reinhardtii

References

Chu, H.-A., Debus, R. J., & Babcock, G. T. (2001). D1-Asp170 Is Structurally Coupled to the Oxygen Evolving Complex in Photosystem II As Revealed by Light-Induced Fourier Transform Infrared Difference Spectroscopy†. Biochemistry, 40(7), 2312-2316. doi:10.1021/bi0022994

Erickson, J. M., Pfister, K., Rahire, M., Togasaki, R. K., Mets, L., & Rochaix, J. D. (1989). Molecular and biophysical analysis of herbicide-resistant mutants of Chlamydomonas reinhardtii: structure-function relationship of the photosystem II D1 polypeptide. The Plant Cell, 1(3), 361-371.

Ferreira, K. N., Iverson, T. M., Maghlaoui, K., Barber, J., & Iwata, S. (2004). Architecture of the photosynthetic oxygen-evolving center. Science, 303(5665), 1831-1838.

Jansen, M. A. K., Mattoo, A. K., Malkin, S., & Edelman, M. (1993). Direct Demonstration of Binding-Site Competition between Photosystem II Inhibitors at the QB Niche of the D1 Protein. Pesticide Biochemistry and Physiology, 46(1), 78-83. doi:http://dx.doi.org/10.1006/pest.1993.1039

Marder, J., Chapman, D., Telfer, A., Nixon, P., & Barber, J. (1987). Identification of psbA and psbD gene products, D1 and D2, as reaction centre proteins of photosystem 2. Plant molecular biology, 9(4), 325-333.

Nixon, P. J., & Diner, B. A. (1992). Aspartate 170 of the photosystem II reaction center polypeptide D1 is involved in the assembly of the oxygen-evolving manganese cluster. Biochemistry, 31(3), 942-948. doi:10.1021/bi00118a041