Difference between revisions of "Part:BBa K1742000:Design"

 
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The LOv2 domain was fused to a small peptide epitope to the C-terminus of the J-alpha helix (-KAVDTWV). Depending on this peptide, the engineered variants of the Erbin PDZ domains would vary in interaction affinity.  
+
The LOv2 domain was fused to a small peptide epitope to the C-terminus of the J-alpha helix (-KAVDTWV). Depending on the nature of this peptide tag, its ligand ePDZ interacts with different interaction affinities.  
−
 
+
The AsLOVpep domain also contains a linker 'GGSGGS-' in the N-terminus
−
 
+
  
 
===Source===
 
===Source===

Revision as of 18:42, 14 September 2015


Avena sativa LOV2 domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The LOv2 domain was fused to a small peptide epitope to the C-terminus of the J-alpha helix (-KAVDTWV). Depending on the nature of this peptide tag, its ligand ePDZ interacts with different interaction affinities. The AsLOVpep domain also contains a linker 'GGSGGS-' in the N-terminus

Source

The AsLOV2 fused to a peptide epitope was designed according to the second LOV domain of Avena sativa phototropin (UniprotKB 049003)

References