Difference between revisions of "Part:BBa K1707013:Design"

 
 
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===Design Notes===
 
===Design Notes===
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During the design, we had to make sure that the RBS included in the RNA thermometer would be at a correct location in front of the cI857 gene when we will assemble the parts. Luckily, the RNA thermometer part (K115017) had been designed to allow the assembly with protein coding part.
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During the construction, we had to introduce the RNA thermometer using Gibson assembly as we saw when we sequenced this part that the cloning of K115017 had failed.  
  
  

Latest revision as of 14:54, 14 September 2015

cI857 under the control of RNA thermometer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During the construction, we had to introduce the RNA thermometer using Gibson assembly as we saw when we sequenced this part that the cloning of K115017 had failed.


Source

This part is the result of the assembly of existing parts.

References