Difference between revisions of "Part:BBa K1864001"
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We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours | We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours | ||
[[File:FAFU-CHINA PART (1).png]] | [[File:FAFU-CHINA PART (1).png]] | ||
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<h3>The inductive effect of different time length</h3> | <h3>The inductive effect of different time length</h3> | ||
We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration. | We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration. | ||
[[File:FAFU-CHINA PART (2).png]] | [[File:FAFU-CHINA PART (2).png]] | ||
Revision as of 10:22, 14 September 2015
T7 RNA Polymerase
T7 RNA polymerase is used to combine with T7 promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1693
IPTG inductive effect
We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.
The inductive effect of different concentration IPTG
We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours
.png)
The inductive effect of different time length
We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration.
.png)