Difference between revisions of "Part:BBa K1595029:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1595029 short</partinfo> | <partinfo>BBa_K1595029 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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Use in conjunction with strain-expressing T7 polymerase. | Use in conjunction with strain-expressing T7 polymerase. | ||
Added GSG so EDA could easily be linked to it's associated peptide in future use. | Added GSG so EDA could easily be linked to it's associated peptide in future use. | ||
| − | + | EDA had an internal PstI cutsite that was removed by altering the DNA sequence. | |
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
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| + | EDA: NCBI accession number P0A955 | ||
Latest revision as of 04:23, 14 September 2015
EDA and GSG linker
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 729
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 641
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Use in conjunction with strain-expressing T7 polymerase.
Added GSG so EDA could easily be linked to it's associated peptide in future use.
EDA had an internal PstI cutsite that was removed by altering the DNA sequence.
Source
Synthesized by DNA 2.0
References
EDA: NCBI accession number P0A955