Difference between revisions of "Part:BBa K1819001"
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It contains an oxidized heme-Fe<sup>3+</sup> not bound to dioxygen.
 
It contains an oxidized heme-Fe<sup>3+</sup> not bound to dioxygen.
  
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http://2015.igem.org/File:Brasil-USPLcpscheme.png
  
  

Revision as of 03:16, 14 September 2015

Latex Clearing Protein (Lcp) - rubber degradation

Latex clearing protein (Lcp) from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1) is a 42kDa enzyme which catalyzes rubber cleavage at the cis double bonds to multiple products ranging from C20 tetra-isoprenoid to at least C35 hepta-isoprenoid with measured specific activity of 1.3 mol/min per mg.

It contains an oxidized heme-Fe3+ not bound to dioxygen.

http://2015.igem.org/File:Brasil-USPLcpscheme.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 889
    Illegal XhoI site found at 1043
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 535
    Illegal NgoMIV site found at 857
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 438