Difference between revisions of "Part:BBa K1819001:Design"

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===Source===
 
===Source===
  
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<partinfo>K1819001 source</partinfo>
 
  
 
  Lcp from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1).
 
  Lcp from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1).

Latest revision as of 03:05, 14 September 2015

Latex Clearing Protein (Lcp) - rubber degradation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 889
    Illegal XhoI site found at 1043
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 535
    Illegal NgoMIV site found at 857
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 438


Design Notes

Because Lcp is secreted by Streptomyces sp, it contains a 5’-signal peptide that was identified using signal peptide predictor server.[1] We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites

Source

Lcp from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1).

References

1. Bagos PG, Nikolaou EP, Liakopoulos TD, Tsirigos KD. Combined prediction of Tat and Sec signal peptides with Hidden Markov Models. Bioinformatics. 2010; 6(22):2811-2817.