Difference between revisions of "Part:BBa K1819001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Because Lcp is secreted by Streptomyces sp, it contains a 5’-signal peptide that was identified using signal peptide predictor server [http://www.compgen.org/tools/PRED-TAT].[1] We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites | + | Because Lcp is secreted by Streptomyces sp, it contains a 5’-signal peptide that was identified using signal peptide predictor server [(http://www.compgen.org/tools/PRED-TAT)].[1] We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites |
===Source=== | ===Source=== | ||
Revision as of 02:57, 14 September 2015
Latex Clearing Protein (Lcp) - rubber degradation
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 889
Illegal XhoI site found at 1043 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 535
Illegal NgoMIV site found at 857 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 438
Design Notes
Because Lcp is secreted by Streptomyces sp, it contains a 5’-signal peptide that was identified using signal peptide predictor server [(http://www.compgen.org/tools/PRED-TAT)].[1] We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites
Source
References
1 Bagos PG, Nikolaou EP, Liakopoulos TD, Tsirigos KD. Combined prediction of Tat and Sec signal peptides with Hidden Markov Models. Bioinformatics. 2010; 6(22):2811-2817.