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Revision as of 23:07, 13 September 2015

PBAD/araC_rbs_fimE(wild-type)

Materials and Methods

1. Construction
All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.

A. PBAD/araC_FimE(6A1) +Fimswitch[default ON]_gfp(3K3)
B. PBAD/araC_FimE(6A1) +Fimswitch[default ON]_gfp(3K3)
C. rbs_M256IcysE(6A1) + Fimswitch[ON]_gfp(3K3) …positive control 1
D. rbs_M256IcysE(6A1) + Fimswitch[OFF]_gfp(3K3) …negative control 1
E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2
F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2

Fig. 1. Plasmids

2. Assay protocol