Difference between revisions of "Part:BBa K1632013:Experience"
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===Materials and Methods=== | ===Materials and Methods=== | ||
<b>1. Construction</b><br> | <b>1. Construction</b><br> | ||
Revision as of 23:07, 13 September 2015
J23100_rbs_rhlR_TT_Plux_rbs_CmRssrA
Contents
Materials and Methods
1. Construction
All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.
A. PBAD/araC_FimE(6A1) +Fimswitch[default ON]_gfp(3K3)
B. PBAD/araC_FimE(6A1) +Fimswitch[default ON]_gfp(3K3)
C. rbs_M256IcysE(6A1) + Fimswitch[ON]_gfp(3K3) …positive control 1
D. rbs_M256IcysE(6A1) + Fimswitch[OFF]_gfp(3K3) …negative control 1
E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2
F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2
2. Assay protocol