Difference between revisions of "Part:BBa K1682000"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1682000 short</partinfo>
 
<partinfo>BBa_K1682000 short</partinfo>
  
−
n/a
+
=Wild Type P<sub>kdpF</sub> - potassium responsive promoter=
  
−
<!-- Add more about the biology of this part here
+
===Biology of P<sub>kdpF</sub>===
−
===Usage and Biology===
+
  
 +
The potassium ion uptake in E. coli is regulated by several systems under different conditions. The potassium ion transporters, Trk and Kup are constitutively expressed (Epstein & Kim, 1971) while KdpFABC, another transporter is activated under low [K+] conditions (Laimins et al., 1981).
 +
[[Image:HKUST-Rice_2015_K_MECHANISM2.jpg|300px|right]]
 +
The kdpFABC operon is controlled by the KdpDE two-component system (TCS) which consists of KdpD, a membrane-bound sensor kinase, and KdpE, a cytoplasmic response regulator (Polarek, 1992; Walderhaug, 1992). KdpD is stimulated by both intracellular and extracellular K+ (Jung, 2000; Jung, 2001; Roe, 2000; Yan, 2011a; Laermann, 2013). KdpD phosphorylates KdpE upon low potassium concentration (Voelkner, 1993; Puppe, 1996; Jung, 1997a; Jung, 2000). Under an increase in [K+], KdpD phosphatase activity will be enhanced, causing a decrease in phospho-KdpE and kdpFABC expression. Phosphorylated KdpE turns on the expression of kdpFABC (Zhang, 2014a; Laermann, 2013).
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 18:12, 13 September 2015

PkdpF

Wild Type PkdpF - potassium responsive promoter

Biology of PkdpF

The potassium ion uptake in E. coli is regulated by several systems under different conditions. The potassium ion transporters, Trk and Kup are constitutively expressed (Epstein & Kim, 1971) while KdpFABC, another transporter is activated under low [K+] conditions (Laimins et al., 1981).

HKUST-Rice 2015 K MECHANISM2.jpg

The kdpFABC operon is controlled by the KdpDE two-component system (TCS) which consists of KdpD, a membrane-bound sensor kinase, and KdpE, a cytoplasmic response regulator (Polarek, 1992; Walderhaug, 1992). KdpD is stimulated by both intracellular and extracellular K+ (Jung, 2000; Jung, 2001; Roe, 2000; Yan, 2011a; Laermann, 2013). KdpD phosphorylates KdpE upon low potassium concentration (Voelkner, 1993; Puppe, 1996; Jung, 1997a; Jung, 2000). Under an increase in [K+], KdpD phosphatase activity will be enhanced, causing a decrease in phospho-KdpE and kdpFABC expression. Phosphorylated KdpE turns on the expression of kdpFABC (Zhang, 2014a; Laermann, 2013). Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 61
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 61
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 61
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 61
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]