Difference between revisions of "Part:BBa K1628303"
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<partinfo>BBa_K1628303 short</partinfo> | <partinfo>BBa_K1628303 short</partinfo> | ||
| − | P3-GFP | + | P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry. |
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Latest revision as of 15:55, 13 September 2015
P3-GFP
P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705