Difference between revisions of "Part:BBa K1628002"

Revision as of 14:25, 13 September 2015

Pxyl

Promoter Pxyl is a promoter of xylose operon regulate by repressor XylR. Promoter Pxyl together with promoter PlacI, repressor LacI (BBa_K1628201), promoter Pgrac (BBa_K1628202) and repressor XylR (BBa_K1628203) formed a metabolic toggle switch. We used this device to regulate the expression of odhAB genes in B. amyloliquefaciens NK-1 (showed in Figure 2). Without IPTG, the promoter Pgrac is inhibited by suppressor LacI and the supreessor XylR will not synthesized, thus the promoter Pxyl is active and odhAB genes are expressed. When IPTG is added, the xylR gene is expressed and the suppressor XylR is synthesized thereafter inhibited the expression of odhAB genes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 Promoter Pxyl is a promoter of xylose operon regulate by repressor XylR. Promoter Pxyl together with promoter PlacI, repressor LacI (BBa_K1628201), promoter Pgrac (BBa_K1628202) and repressor XylR (BBa_K1628203) formed a metabolic toggle switch. We used this device to regulate the expression of ''odhAB'' genes in ''B. amyloliquefaciens'' NK-1 (showed in Figure 2).
+Without IPTG, the promoter Pgrac is inhibited by suppressor LacI and the supreessor XylR will not synthesized, thus the promoter Pxyl is active and ''odhAB'' genes are expressed. When IPTG is added, the ''xylR'' gene is expressed and the suppressor XylR is synthesized thereafter inhibited the expression of ''odhAB'' genes.
 [[File:Pxyl_NK1.png]]