Difference between revisions of "Part:BBa K1632013:Design"

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===References===
 
===References===
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''Timothy S. Ham et al''. (2006) A Tightly Regulated Inducible Expression System Utilizing the fim Inversion Recombination Switch. Biotechnol Bioeng 94(1):1-4

Revision as of 14:02, 13 September 2015

PBAD/araC_rbs_fimE(wild-type)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1260
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Materials and Methods

1. Construction
All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.

A. PBAD/araC_FimE(6A1) +Fimswitch[default ON]_gfp(3K3)
B. PBAD/araC_FimE(6A1) +Fimswitch[default ON]_gfp(3K3)
C. rbs_M256IcysE(6A1) + Fimswitch[ON]_gfp(3K3) …positive control 1
D. rbs_M256IcysE(6A1) + Fimswitch[OFF]_gfp(3K3) …negative control 1
E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2
F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2

Fig. 1. Plasmids

2. Assay protocol

Design Notes

sequence confirmed

Materials and Methods

Source

Composite of BBa_I0500, BBa_B0034, BBa_K1632011

References

Timothy S. Ham et al. (2006) A Tightly Regulated Inducible Expression System Utilizing the fim Inversion Recombination Switch. Biotechnol Bioeng 94(1):1-4