Difference between revisions of "Part:BBa K1699003:Design"

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IDT de-novo synthesis.
 
IDT de-novo synthesis.
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===References===
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1. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
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<br />http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full
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2. Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas
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<br />http://pubs.acs.org/doi/full/10.1021/sb400081r
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3. In vivo genome editing using Staphylococcus aureus Cas9.
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<br />http://10.1038/nature14299

Revision as of 10:34, 13 September 2015

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 144
    Illegal NgoMIV site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned into pSBC13 using EcoRI and PstI restriction sites.
F. tagtcatgGAATTCGCGGCCGCTTCTAG
R. tgtatactCTGCAGCGGCCGCTAC

Source

IDT de-novo synthesis.


References

1. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full

2. Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas
http://pubs.acs.org/doi/full/10.1021/sb400081r

3. In vivo genome editing using Staphylococcus aureus Cas9.
http://10.1038/nature14299