Difference between revisions of "Part:BBa K1699001:Experience"
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This part is an improved version of <a href="https://parts.igem.org/Part:BBa_K404106" target="blank">phTERT-K404106</a>. Being shorter, and more characterized with results of the part being used in human cell lines experiments.
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This part is an improvement  of existing part [https://parts.igem.org/Part:BBa_K404106] due to the following reasons:
  
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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1. Our part is shorter (378 bp vs 459 bp), thus permitting more flexible design in various scenarios when length could be a critical issue.
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how you used this part and how it worked out.
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2. Our part adds extensive characterization results in human cancer cells, showing data of promoter validation, and, importantly, functional data where hTERT promoter was used to successfully control the expression of dCas9-VP64 in cancer-specific CRIPSR-based activation system. In the original part, actual experimental data is missing.
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3. The design summary of hTERT promoter in cancer-specific CRISPR-based gene knock-out is also shown, further expanding the usability of the part.
  
  

Revision as of 10:10, 13 September 2015


This part is an improvement of existing part [1] due to the following reasons:

1. Our part is shorter (378 bp vs 459 bp), thus permitting more flexible design in various scenarios when length could be a critical issue. 2. Our part adds extensive characterization results in human cancer cells, showing data of promoter validation, and, importantly, functional data where hTERT promoter was used to successfully control the expression of dCas9-VP64 in cancer-specific CRIPSR-based activation system. In the original part, actual experimental data is missing. 3. The design summary of hTERT promoter in cancer-specific CRISPR-based gene knock-out is also shown, further expanding the usability of the part.


Applications of BBa_K1699001

User Reviews

UNIQc35733f4e5cfefc1-partinfo-00000000-QINU UNIQc35733f4e5cfefc1-partinfo-00000001-QINU