Difference between revisions of "Part:BBa K1864001"
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<h3>Concentration of dsRdRp differs under different concentration of IPTG</h3>
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<h3>The inductive effect of different concentration IPTG</h3>
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<b>The concentration of dsRdRp continues to increase till the concentration of IPTG reaches to 0.4 mmol/L.<br />
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<b>We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours <br />
 
[[File:FAFU-CHINA PART (1).png]]
 
[[File:FAFU-CHINA PART (1).png]]

Revision as of 09:02, 13 September 2015

T7 RNA Polymerase

T7 RNA polymerase is used to combine with T7 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1693


IPTG inductive effect

We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.


The inductive effect of different concentration IPTG

We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours
FAFU-CHINA PART (1).png