Difference between revisions of "Part:BBa K1864001"
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We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect. | We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect. | ||
</p> | </p> | ||
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| + | <h3>Concentration of dsRdRp differs under different concentration of IPTG</h3> | ||
| + | <b>The concentration of dsRdRp continues to increase till the concentration of IPTG reaches to 0.4 mmol/L.<br /> | ||
| + | <b>Millevoi, S., Loulergue, C., Dettwiler, S., Karaa, S. Z., Keller, W., Antoniou, M., et al.</b> (2006). An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries. The EMBO journal, 25(20), 4854-64. doi: 10.1038/sj.emboj.7601331. | ||
| + | [[File:FAFU-CHINA PART (1).png]] | ||
Revision as of 08:52, 13 September 2015
T7 RNA Polymerase
T7 RNA polymerase is used to combine with T7 promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1693
IPTG inductive effect
We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.
Concentration of dsRdRp differs under different concentration of IPTG
The concentration of dsRdRp continues to increase till the concentration of IPTG reaches to 0.4 mmol/L.
<b>Millevoi, S., Loulergue, C., Dettwiler, S., Karaa, S. Z., Keller, W., Antoniou, M., et al. (2006). An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries. The EMBO journal, 25(20), 4854-64. doi: 10.1038/sj.emboj.7601331.
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