Difference between revisions of "Part:BBa K1659200:Design"
Weikongquee (Talk | contribs) |
|||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1659200 short</partinfo> | <partinfo>BBa_K1659200 short</partinfo> | ||
Line 6: | Line 5: | ||
− | ===Design Notes=== | + | ===Design Notes and Sources=== |
− | + | ||
+ | A Hisx6 tag is added at the C-terminus for ease of protein purification using metal-affinity chromatography. | ||
+ | We obtained the peptide sequence for DspB from Kaplan et al's original publication and converted it into a nucleotide sequence using [https://www.idtdna.com/CodonOpt IDT's Codon Optimizer], optimizing based on ''E. coli'' K-12's codon table [1]. | ||
− | |||
− | |||
===References=== | ===References=== | ||
+ | |||
+ | [1] Kaplan, J.B. et al., 2004. Genes Involved in the Synthesis and Degradation of Matrix Polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae Biofilms. Journal of Bacteriology, 186(24), pp.8213–8220. |
Latest revision as of 23:30, 12 September 2015
Dispersin B, an antibiofilm enzyme that specifically hydrolyses PGA polymers
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 262
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 418
Design Notes and Sources
A Hisx6 tag is added at the C-terminus for ease of protein purification using metal-affinity chromatography.
We obtained the peptide sequence for DspB from Kaplan et al's original publication and converted it into a nucleotide sequence using IDT's Codon Optimizer, optimizing based on E. coli K-12's codon table [1].
References
[1] Kaplan, J.B. et al., 2004. Genes Involved in the Synthesis and Degradation of Matrix Polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae Biofilms. Journal of Bacteriology, 186(24), pp.8213–8220.