Difference between revisions of "Part:BBa M31022:Design"
Line 1: Line 1:
−
 
−
__NOTOC__
 
−
<partinfo>BBa_M31022 short</partinfo>
 
−
 
−
<partinfo>BBa_M31022 SequenceAndFeatures</partinfo>
 
−
 
−
 
−
===Design Notes===
 
 
<tt>
 
<tt>
−
I wanted to weaken RBS X and preserve II AA sequence.
 
  
 
II:              gca ttt gag ggg gat tca
 
II:              gca ttt gag ggg gat tca
 
X RBS:            gca ttt gag ggg gat tca
 
X RBS:            gca ttt gag ggg gat tca
 
AA seq:          ALA PHE GLU GLY ASP SER
 
AA seq:          ALA PHE GLU GLY ASP SER
−
 
+
                        -10^
−
                        -10^
+
 
X.1 RBS region:  gcg ttc gaa ggc gac tcg
 
X.1 RBS region:  gcg ttc gaa ggc gac tcg
 
New AA seq:      ALA PHE GLU GLY ASP SER
 
New AA seq:      ALA PHE GLU GLY ASP SER
−
 
−
 
−
 
−
 
−
===Source===
 
−
 
−
gene II
 
−
 
−
===References===
 

Revision as of 08:55, 28 February 2007

II: gca ttt gag ggg gat tca X RBS: gca ttt gag ggg gat tca AA seq: ALA PHE GLU GLY ASP SER 

                        -10^

X.1 RBS region: gcg ttc gaa ggc gac tcg New AA seq: ALA PHE GLU GLY ASP SER