Difference between revisions of "Part:BBa K1659200"
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<partinfo>BBa_K1659200 short</partinfo> | <partinfo>BBa_K1659200 short</partinfo> | ||
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| − | + | This part contains the sequence for the biofilm-degrading enzyme, Dispersin B. | |
| − | ===Usage | + | |
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| + | ===Biology=== | ||
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| + | Dispersin B is an enzyme produced by ''Aggregatibacter actinomycetemcomitans'', a species of bacteria found in the human oral cavity that grows almost exclusively in the form of biofilms. ''A. actinomycetemcomitans'' uses Dispersin B as a means of spreading its colonies by degrading a portion of its mature biofilm and releasing cells that were previously adherent, allowing them to propagate through liquid medium a form new biofilms on other surfaces. Kaplan et al identified the gene that coded for Dispersin B and characterized the protein using ''E. coli'' as the expression host [1]. | ||
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| + | Structural analysis of Dispersin B showed that the enzyme only works specifically against the β-1,6-glycosidic linkages found in poly-N-acetylglucosamine (PGA), which is a polysaccharide structural element found in the biofilms of ''E. coli'', ''S. aureus'', and ''S. epidermidis'', but not in ''P. aeruginosa'' [2][3][4]. | ||
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| + | Dispersin B is currently patented and licensed to Kane Biotech which is developing a wound care spray based on it [5][6]. When used in conjunction with triclosan as a surface prophylactic agent, Dispersin B effectively inhibited the formation of biofilms on both the internal and external surfaces of urinary catheters [7]. | ||
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| + | ===Usage=== | ||
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| + | ===References=== | ||
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| + | [1] Kaplan, J.B. et al., 2003. Detachment of Actinobacillus actinomycetemcomitans Biofilm Cells by an Endogenous beta-Hexosaminidase Activity. Journal of Bacteriology, 185(16), pp.4693–4698. | ||
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| + | [2] Ramasubbu, N. et al., 2005. Structural analysis of dispersin B, a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans. Journal of Molecular Biology, 349, pp.475–486. | ||
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| + | [3] Manuel, S.G. a et al., 2007. Role of active-site residues of dispersin B, a biofilm-releasing beta-hexosaminidase from a periodontal pathogen, in substrate hydrolysis. FEBS Journal, 274(22), pp.5987–5999. | ||
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| + | [4] Wang, X., Iii, J.F.P. & Romeo, T., 2004. The pgaABCD Locus of Escherichia coli Promotes the Synthesis of a Polysaccharide Adhesin Required for Biofilm Formation. Journal of Bacteriology, 186(9), pp.2724–2734. | ||
| − | + | [5] University Of Medicine And Dentistry Of New Jersey, (2011). Dispersin B polynucleotides and methods of producing recombinant DspB polypeptides. US7989604 B2. | |
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| + | [6] Kane Biotech, 2011. Access online at: http://www.kanebiotech.com/dispersinb.html | ||
| − | + | [7] Darouiche, R.O. et al., 2009. Antimicrobial and antibiofilm efficacy of triclosan and DispersinB combination. Journal of Antimicrobial Chemotherapy, 64(May), pp.88–93. | |
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Revision as of 18:36, 12 September 2015
Dispersin B, an antibiofilm enzyme that specifically hydrolyses PGA polymers
This part contains the sequence for the biofilm-degrading enzyme, Dispersin B.
Biology
Dispersin B is an enzyme produced by Aggregatibacter actinomycetemcomitans, a species of bacteria found in the human oral cavity that grows almost exclusively in the form of biofilms. A. actinomycetemcomitans uses Dispersin B as a means of spreading its colonies by degrading a portion of its mature biofilm and releasing cells that were previously adherent, allowing them to propagate through liquid medium a form new biofilms on other surfaces. Kaplan et al identified the gene that coded for Dispersin B and characterized the protein using E. coli as the expression host [1].
Structural analysis of Dispersin B showed that the enzyme only works specifically against the β-1,6-glycosidic linkages found in poly-N-acetylglucosamine (PGA), which is a polysaccharide structural element found in the biofilms of E. coli, S. aureus, and S. epidermidis, but not in P. aeruginosa [2][3][4].
Dispersin B is currently patented and licensed to Kane Biotech which is developing a wound care spray based on it [5][6]. When used in conjunction with triclosan as a surface prophylactic agent, Dispersin B effectively inhibited the formation of biofilms on both the internal and external surfaces of urinary catheters [7].
Usage
References
[1] Kaplan, J.B. et al., 2003. Detachment of Actinobacillus actinomycetemcomitans Biofilm Cells by an Endogenous beta-Hexosaminidase Activity. Journal of Bacteriology, 185(16), pp.4693–4698.
[2] Ramasubbu, N. et al., 2005. Structural analysis of dispersin B, a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans. Journal of Molecular Biology, 349, pp.475–486.
[3] Manuel, S.G. a et al., 2007. Role of active-site residues of dispersin B, a biofilm-releasing beta-hexosaminidase from a periodontal pathogen, in substrate hydrolysis. FEBS Journal, 274(22), pp.5987–5999.
[4] Wang, X., Iii, J.F.P. & Romeo, T., 2004. The pgaABCD Locus of Escherichia coli Promotes the Synthesis of a Polysaccharide Adhesin Required for Biofilm Formation. Journal of Bacteriology, 186(9), pp.2724–2734.
[5] University Of Medicine And Dentistry Of New Jersey, (2011). Dispersin B polynucleotides and methods of producing recombinant DspB polypeptides. US7989604 B2.
[6] Kane Biotech, 2011. Access online at: http://www.kanebiotech.com/dispersinb.html
[7] Darouiche, R.O. et al., 2009. Antimicrobial and antibiofilm efficacy of triclosan and DispersinB combination. Journal of Antimicrobial Chemotherapy, 64(May), pp.88–93.