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| CrdA might assist translocation of the nascent polymer of Curdlan across the cytoplasmic membrane. | | CrdA might assist translocation of the nascent polymer of Curdlan across the cytoplasmic membrane. |
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− | ===Usage and Biology===
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− | <p align="justify">
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− | Curdlan is a bacterial polysaccharide (linear (1->3)-β-glucan) that has been of significant recent interest due to its valuable properties and its bioactivity.
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− | In fact, Curdlan belongs to the class of biological response modifiers that enhance or restore normal immune defenses. For example, it can have antitumor, anti-infective, anti-inflammatory, and anticoagulant activities (see other properties of Curdlan). In particular, this (1->3)-β-glucan can stimulate plants' immune system.
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− | <br>Our project is focused on the production of curdlan for stimulate plant defense system against Downy Mildew.
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− | </p>
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− | The molecular genetics of curdlan production have been investigated in ''Agrobacterium sp.'' ATCC31749.
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− | Studies identified three genes (''crdA'', ''crdS'' and ''crdC'') that are essential for curdlan production
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− | The ''crdASC'' genes occupy a contiguous 4,948 bp region of the genome ( the crdS gene is flanked by the ''crdA'' and ''crdC'' genes).
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− | </p>
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− | <span class='h3bb'>Sequence and Features</span>
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− | <partinfo>BBa_K1686045 SequenceAndFeatures</partinfo>
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− | For our project, we wanted to produce curdlan by ''E.coli'', so this part has been made with a codon optimization of the natural crdS sequence for ''E.coli''.
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− | </p>
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− | <!-- Uncomment this to enable Functional Parameter display
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− | ===Functional Parameters===
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− | <partinfo>BBa_K1686045 parameters</partinfo>
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Revision as of 15:37, 12 September 2015
crdA gene with codon optimisation for E. coli
CrdA might assist translocation of the nascent polymer of Curdlan across the cytoplasmic membrane.