Difference between revisions of "Part:BBa K1686045:Design"
 
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<partinfo>BBa_K1686046 short</partinfo>
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<partinfo>BBa_K1686045 short</partinfo>
  
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<partinfo>BBa_K1686046 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1686045 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
  
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The gene of Curdlan synthase comes from ''Agrobacterium sp.'' ATCC31749 and we would like to use it in ''E.coli'', so we fixed the bias codon by using the software of ''Integrated DNA Technologies, Inc'' for codon optimization.
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The gene of CrdA comes from ''Agrobacterium sp.'' ATCC31749 and we would like to use it in ''E.coli'', so we fixed the bias codon by using the software of ''Integrated DNA Technologies, Inc'' for codon optimization.
  
  
 
===Source===
 
===Source===
  
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The nucleotide sequence of crdS comes from GenBank under the accession number AECL01000028.1
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The nucleotide sequence of CrdA comes from GenBank under the accession number AECL01000028.1
  
  

Latest revision as of 14:13, 12 September 2015

crdA gene with codon optimisation for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 399
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 859
    Illegal AgeI site found at 828
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene of CrdA comes from Agrobacterium sp. ATCC31749 and we would like to use it in E.coli, so we fixed the bias codon by using the software of Integrated DNA Technologies, Inc for codon optimization.


Source

The nucleotide sequence of CrdA comes from GenBank under the accession number AECL01000028.1


References

Stasinopoulos SJ, et al. (1999). "Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene." Glycobiology 9:31–41.