Difference between revisions of "Part:BBa K1686046"
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In fact, Curdlan belongs to the class of biological response modifiers that enhance or restore normal immune defenses. For example, it can have antitumor, anti-infective, anti-inflammatory, and anticoagulant activities (see other properties of Curdlan). In particular, this (1->3)-β-glucan can stimulate plants' immune system. | In fact, Curdlan belongs to the class of biological response modifiers that enhance or restore normal immune defenses. For example, it can have antitumor, anti-infective, anti-inflammatory, and anticoagulant activities (see other properties of Curdlan). In particular, this (1->3)-β-glucan can stimulate plants' immune system. | ||
<br>Our project is focused on the production of curdlan for stimulate plant defense system against Downy Mildew. | <br>Our project is focused on the production of curdlan for stimulate plant defense system against Downy Mildew. | ||
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The molecular genetics of curdlan production have been investigated in ''Agrobacterium sp.'' ATCC31749. | The molecular genetics of curdlan production have been investigated in ''Agrobacterium sp.'' ATCC31749. | ||
Studies identified three genes (''crdA'', ''crdS'' and ''crdC'') that are essential for curdlan production | Studies identified three genes (''crdA'', ''crdS'' and ''crdC'') that are essential for curdlan production | ||
The ''crdASC'' genes occupy a contiguous 4,948 bp region of the genome ( the crdS gene is flanked by the ''crdA'' and ''crdC'' genes). | The ''crdASC'' genes occupy a contiguous 4,948 bp region of the genome ( the crdS gene is flanked by the ''crdA'' and ''crdC'' genes). | ||
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The ''crdS'' gene (1965 bp) encodes the curdlan synthase (CrdS, 73 kDa) protein. | The ''crdS'' gene (1965 bp) encodes the curdlan synthase (CrdS, 73 kDa) protein. | ||
In ''Agrobacterium'', CrdS is an integral inner membrane protein with seven transmembrane (TM) helices, one non-membrane-spanning amphipathic helix and a N<sub>out</sub>–C<sub>in</sub> disposition. | In ''Agrobacterium'', CrdS is an integral inner membrane protein with seven transmembrane (TM) helices, one non-membrane-spanning amphipathic helix and a N<sub>out</sub>–C<sub>in</sub> disposition. | ||
The UDP-Glucose substrate-binding and the catalytic motif for curdlan polymerisation is observed between TM3 and TM4. | The UDP-Glucose substrate-binding and the catalytic motif for curdlan polymerisation is observed between TM3 and TM4. | ||
The process of Curdlan polymerisation mediated by CrdS occurs on the cytoplasmic face of the inner membrane | The process of Curdlan polymerisation mediated by CrdS occurs on the cytoplasmic face of the inner membrane | ||
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For our project, we wanted to produce curdlan by ''E.coli'', so this part has been made with a codon optimization of the natural crdS sequence for ''E.coli''. | For our project, we wanted to produce curdlan by ''E.coli'', so this part has been made with a codon optimization of the natural crdS sequence for ''E.coli''. | ||
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Revision as of 13:47, 12 September 2015
Curdlan synthase gene with codon optimisation for E. coli
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 801
Illegal AgeI site found at 1192 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Curdlan is a bacterial polysaccharide (linear (1->3)-β-glucan) that has been of significant recent interest due to its valuable properties and its bioactivity.
In fact, Curdlan belongs to the class of biological response modifiers that enhance or restore normal immune defenses. For example, it can have antitumor, anti-infective, anti-inflammatory, and anticoagulant activities (see other properties of Curdlan). In particular, this (1->3)-β-glucan can stimulate plants' immune system.
Our project is focused on the production of curdlan for stimulate plant defense system against Downy Mildew.
The molecular genetics of curdlan production have been investigated in Agrobacterium sp. ATCC31749. Studies identified three genes (crdA, crdS and crdC) that are essential for curdlan production The crdASC genes occupy a contiguous 4,948 bp region of the genome ( the crdS gene is flanked by the crdA and crdC genes).
The crdS gene (1965 bp) encodes the curdlan synthase (CrdS, 73 kDa) protein. In Agrobacterium, CrdS is an integral inner membrane protein with seven transmembrane (TM) helices, one non-membrane-spanning amphipathic helix and a Nout–Cin disposition. The UDP-Glucose substrate-binding and the catalytic motif for curdlan polymerisation is observed between TM3 and TM4. The process of Curdlan polymerisation mediated by CrdS occurs on the cytoplasmic face of the inner membrane
For our project, we wanted to produce curdlan by E.coli, so this part has been made with a codon optimization of the natural crdS sequence for E.coli.