Difference between revisions of "Part:BBa K1686046:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The gene of Curdlan synthase comes from Agrobacterium and we would like to use it in E.coli, so we fixed the bias codon by using the software of ''Integrated DNA Technologies, Inc'' for codon optimization. | + | The gene of Curdlan synthase comes from Agrobacterium and we would like to use it in ''E.coli'', so we fixed the bias codon by using the software of ''Integrated DNA Technologies, Inc'' for codon optimization. |
===Source=== | ===Source=== | ||
| − | |||
The nucleotide sequence of crdS comes from GenBank under the accession number AF057142.2 | The nucleotide sequence of crdS comes from GenBank under the accession number AF057142.2 | ||
Revision as of 13:40, 12 September 2015
Curdlan synthase gene with codon optimisation for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 678
Illegal XhoI site found at 400 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 801
Illegal AgeI site found at 1192 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene of Curdlan synthase comes from Agrobacterium and we would like to use it in E.coli, so we fixed the bias codon by using the software of Integrated DNA Technologies, Inc for codon optimization.
Source
The nucleotide sequence of crdS comes from GenBank under the accession number AF057142.2
References
Stasinopoulos SJ, et al. (1999). "Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene." Glycobiology 9:31–41.