Difference between revisions of "Part:BBa K1632011:Design"
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| − | + | 2008_Caltech FimE(BBa_K137007) didn’t match the nucleotide sequence of fimE of wild type. So, we designed FimE (BBa_K1632011) which completely match the nucleotide sequence of fimE of wild type. Compared with these two parts, two differences were confirmed. First, fimE of 2008_Caltech lacks the nucleotide sequence of N-terminal 15 residues. Second, fimE of 2008_Caltech is different from the nucleotide sequence of C-terminal 2 residues as shown as below.<br> | |
2008_Caltech : 5’-...-TAA-TAA-Suffix-3’<br> | 2008_Caltech : 5’-...-TAA-TAA-Suffix-3’<br> | ||
2015_TokyoTech : 5’-...-GTT-TGA-Suffix-3’<br> | 2015_TokyoTech : 5’-...-GTT-TGA-Suffix-3’<br> | ||
Revision as of 13:30, 12 September 2015
fimE (wild-type)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 22
- 1000COMPATIBLE WITH RFC[1000]
2008_Caltech FimE(BBa_K137007) didn’t match the nucleotide sequence of fimE of wild type. So, we designed FimE (BBa_K1632011) which completely match the nucleotide sequence of fimE of wild type. Compared with these two parts, two differences were confirmed. First, fimE of 2008_Caltech lacks the nucleotide sequence of N-terminal 15 residues. Second, fimE of 2008_Caltech is different from the nucleotide sequence of C-terminal 2 residues as shown as below.
2008_Caltech : 5’-...-TAA-TAA-Suffix-3’
2015_TokyoTech : 5’-...-GTT-TGA-Suffix-3’
Design Notes
sequence confirmed
Materials and Methods
Source
PCR from MG1655