Difference between revisions of "Part:BBa K1606007"

Latest revision as of 00:11, 12 September 2015

Kumamax enzyme that gluten updated m-cherry reporter device

We modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment. Besides LacI promoter works under acidic environment in the dough formation.

mCherry(restricted)-GFP-GFP

101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherry

101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherryLAST.PNG

KumamolisinMAX-strain3b-KumamolisinMAX-stain4a-2(compability)-mCherry(nice)-1(compability)-mCherry-mCherry

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 2646
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1397
Illegal NgoMIV site found at 1670
Illegal NgoMIV site found at 2198
Illegal NgoMIV site found at 2444
Illegal AgeI site found at 793
Illegal AgeI site found at 905
Illegal AgeI site found at 1722
Illegal AgeI site found at 2298
Illegal AgeI site found at 2538
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
-<h2 align=center>mCherry(restricted)-GFP-GFP-PGap-z</h2>
+<h2 align=center>mCherry(restricted)-GFP-GFP</h2>
 https://static.igem.org/mediawiki/2015/thumb/9/97/MCherry%28restricted%29-GFP-GFP-PGap-z.JPG/319px-MCherry%28restricted%29-GFP-GFP-PGap-z.JPG
@@ Line 11: / Line 11: @@
 https://static.igem.org/mediawiki/2015/f/fa/101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherryLAST.PNG
+<h2 align=center>KumamolisinMAX-strain3b-KumamolisinMAX-stain4a-2(compability)-mCherry(nice)-1(compability)-mCherry-mCherry</h2>
+https://static.igem.org/mediawiki/2015/0/06/Kumma123metu.PNG
 <!-- Add more about the biology of this part here